• Systems biology of vertebrate development and disease


How do extracellular signaling molecules pattern developing embryos? We combine genetic, biophysical, and theoretical approaches to address this question during vertebrate development.

My group studies how the interplay between signaling molecules controls development and disease. We combine genetic, biophysical, and theoretical approaches to address these questions in vertebrate model systems (zebrafish, mouse embryonic stem cells, and patient-derived cancer cells).

Our research focuses on four areas:

Biophysics of signal dispersal: Multiple signaling pathways need to be precisely coordinated in space and time to pattern the body plan. How are the appropriate distributions of signaling molecules achieved, such that cells receive the right amount of signal at the right time?

Self-organization of patterning: Systems of signals that diffuse and react with each other (reaction-diffusion systems) have been postulated to underlie complex self-organizing patterns. How do signaling systems concertedly coordinate proper patterning?

Scale-invariant patterning: Individuals of the same species can vary considerably in size, but the proportions of their body plans are often constant. How is the spatial range of signaling molecules regulated to establish the correct tissue proportions in differently sized embryos?

Aberrant signaling in disease: Signaling pathways that control embryogenesis are frequently deregulated in human cancers. How do acquired mutations in signaling pathway components lead to disease onset, and how can treatment therapies be tailored to patients?

Dr. Patrick Müller
Working in the FML - Müller Group

We use a systems biology approach to understand self-organizing processes during development and disease.

Dr. Patrick Müller
FML Group Leader

 


Key Publications

  1. Mörsdorf D, Müller P (2019). Tuning protein diffusivity with membrane tethers. Biochemistry, 58:177-181.
  2. Almuedo-Castillo M, Bläßle A, Mörsdorf D, Marcon L, Soh GH, Rogers KW, Schier AF, Müller P (2018). Scale-invariant patterning by size-dependent inhibition of Nodal signalling. Nature Cell Biology, doi: 10.1038/s41556-018-0155-7.
  3. Bläßle A, Soh G, Braun T, Mörsdorf D, Preiß H, Jordan BM, Müller P (2018). Quantitative diffusion measurements using the open-source software PyFRAP. Nature Communications, doi: 10.1038/s41467-018-03975-6.
  4. Pomreinke AP, Soh GH, Rogers KW, Bergmann JK, Bläßle AJ, Müller P (2017). Dynamics of BMP signaling and distribution during zebrafish dorsal-ventral patterning. eLife, doi: 10.7554/eLife.25861.
  5. Marcon L, Diego X, Sharpe J, Müller P (2016). High-throughput mathematical analysis identifies Turing networks for patterning with equally diffusing signals. eLife, doi: 10.7554/eLife.14022.

see full publication list here

Future Plans

Our long-term research goal is to understand how the interplay between signals controls development and disease, to precisely control Nodal and BMP signaling using optogenetics, and to build synthetic self-organizing signaling systems.